Journal: Science Advances
Article Title: Inhibition of acute lung inflammation by a neuroimmune circuit induced by vagal nerve stimulation
doi: 10.1126/sciadv.adw7080
Figure Lengend Snippet: ( A ) Serum TNFα protein detected by ELISA and ( B ) lung mRNA expression of Tnf α by qRT-PCR in WT (C57BL/6N) and β 2 AR KO mice subjected to electrical VNS. ( C ) Serum TNFα protein detected by ELISA and ( D ) lung mRNA expression of Tnf α in mice subjected to intravenous administration of vehicle (water) or β 2 AR antagonist (ICI 118 551) before challenge with R848 and electrical VNS. ( E ) Uniform manifold approximation and projection (UMAP) plot of all lung cells that express the adrb2 , with the highest expression indicated by yellow. Expression of adrb1 , adbr2 , and adrb3 and percent of cells that expressed these genes of interest were evaluated. Populations with higher expression of the β 2 AR are indicated by larger circles and deeper coloring. ( F ) mRNA expression of β 1 AR (left) and β 2 AR (right) by qRT-PCR from CD45 + -enriched BALF cells in WT (C57BL/6N) and β 2 AR KO mice. ( G ) mRNA expression of splenic Tnf α by qRT-PCR. Frequency of TNF + ( H ) splenic marginal metallophilic macrophages (MMMɸ), ( I ) red pulp macrophages (RPMɸ), ( J ) marginal zone macrophages (MZMɸ), and ( K ) pDCs by flow cytometry analysis. ( L ) mRNA expression of the β 2 AR on CD45 + -enriched BALF cells and FACS-sorted splenic pDCs by qRT-PCR. ( M ) mRNA expression of right lung cranial lobe Tnf α in mice treated with vehicle (water) or the β 2 AR agonist salbutamol (Sal, 1 mg/kg, i.v.) before challenge with water or R848. ( N ) Frequency of TNF + alveolar macrophages treated in vitro with R848 +/− salbutamol, isolated from the BALF of WT (C57BL/6N) or β 2 AR KO mice. Data represented as means ± SD. One-way ANOVA was used for statistical analysis followed by post hoc analysis with Tukey’s multiple comparisons test. * P < 0.05, ** P < 0.01, *** P < 0.001, and **** P < 0.0001.
Article Snippet: Selective blockade of β 2 AR receptors was performed with ICI 118 551 (Tocris, Minneapolis, MN): A dose (0.5 mg/kg) or vehicle control (water) was instilled as droplets intranasally 10 min before beginning VNS.
Techniques: Enzyme-linked Immunosorbent Assay, Expressing, Quantitative RT-PCR, Flow Cytometry, In Vitro, Isolation
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